KMID : 0362420080460060620
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Journal of Korean Academy of Prosthodontics 2008 Volume.46 No. 6 p.620 ~ p.627
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Cellular responses on anodized titanium discs coated with 1¥á,25-dihydroxyvitamin D3 incorporated Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles
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Cho Young-Jin
Heo Seong-Joo Koak Jai-Young Kim Seong-Kyun Lee Joo-Hee
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Abstract
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Statement of Problem: A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces.
Purpose: The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/1¥á,25-(OH)2D3 coating with reference to cellular proliferation and differentiation in vitro.
Material and Methods: 96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/1¥á,25-(OH)2D3 solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTSbased cell proliferation assay and ALPase activity test were carried out.
Results: PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated 1 ¥á,25-(OH)2D3 (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05).
Conclusion: Biodegradable PLGA nanoparticles incorporated with vitamin D metabolite positively affected proliferation and differentiation of cells on the anodized titanium surface.
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KEYWORD
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1¥á, 25-dihydroxyvitamin D3, PLGA, Nanoparticle, Anodized titanium, Cell proliferation, Differentiation
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